Improved glutathione production by gene expression in Pichia pastoris
AbstractTo utilize Pichia pastoris to produce glutathione, an intracellular expression vector harboring two genes (gsh1 and gsh2) from Saccharomyces cerevisiae encoding enzymes involved in glutathione...
View ArticleFrom cells to embryos to rooted plantlets in a mist bioreactor
AbstractA mist bioreactor using a disposable bag as culture chamber was used to propagate carrot embryogenic cells into rooted plantlets. The best operating configuration was akin to a vertical hanging...
View ArticleFrom leaf explants to rooted plantlets in a mist reactor
AbstractThe medicinally important plant Artemisia annua was used as an example to study the effect of mist reactor conditions on shooting, rooting, and in vitro acclimatization. During all three...
View ArticleBioreactors for Plant Embryogenesis and Beyond
AbstractA variety of different bioreactors have been developed for use in initiating and cultivating somatic embryos. The various designs for embryogenesis and culture are critically evaluated here....
View ArticleFrom leaf explants to hanging rooted plantlets in a mist reactor
AbstractA mist bioreactor using a disposable bag as a culture chamber was used to propagate single nodal explants of Artemisia annua into rooted plantlets that were ready for soil using a single batch...
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